Coding

Part:BBa_I730000:Design

Designed by: Javier Urchueguía   Group: iGEM07_Valencia   (2007-06-27)

Feruloyl CoA hydratase for vanillin biosynthesis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 811
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 811
    Illegal NotI site found at 755
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 811
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 811
    Illegal NgoMIV site found at 184
    Illegal NgoMIV site found at 202
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

design considerations


Source

Microb Cell Fact. 2007; 6: 13. Published online 2007 April 16. doi: 10.1186/1475-2859-6-13. Copyright © 2007 Barghini et al; licensee BioMed Central Ltd. Vanillin production using metabolically engineered Escherichia coli under non-growing conditions Paolo Barghini,1 Diana Di Gioia,2 Fabio Fava,2 and Maurizio Ruzzicorresponding author1 1Department of Agrobiology and Agrochemistry, University of Tuscia, via Camillo de Lellis – snc, 01100 Viterbo, Italy 2DICASM, Faculty of Engineering, University of Bologna, Bologna, Italy corresponding authorCorresponding author. Paolo Barghini: barghini@unitus.it; Diana Di Gioia: diana.digioia@unibo.it; Fabio Fava: fabio.fava@unibo.it; Maurizio Ruzzi: ruzzi@unitus.it Received December 28, 2006; Accepted April 16, 2007.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

References